BigDye Terminator Removal (500mL)

44.000,00 kr.

BigDye terminator removal sequencing clean up kit

CleanDTR is an efficient paramagnetic bead-based system, designed to remove unincorporated dye terminators from Sanger sequencing reactions. The CleanDTR process involves three simple steps including volume, wash and elute. While binding the sequencing product selectively to the magnetic particles, unincorporated dyes, nucleotides, salts and primers will be removed during ethanol washes. This principle allows for the elution of the pure Sanger Sequencing product in the elution buffer of choice. The protocol can be adapted to your current liquid action workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilising your current protocol as well as it can be performed manually.

Details

SKU CDTR-0500 Categories , Tag

Features

  • Long Phred 20 read lengths averaging over 800 bps
  • Pass rates over 85% or higher
  • Efficient elimination of sequencing reaction contaminants
  • Reduce BigDye* usage, dove to increased average signal strength

Applications:

Clean up of sequencing product for both ABI and MegaBACE platforms

Supported Chemistries:

  • BigDye* versions 1.0, 1.1, 2.0, 3.0 and 3.1
  • DYEnamic ET

* BigDye is a registered trademark of Applied Biosystems

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